Cell Line Description

Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.

Components

ATG002A-1VL: A549 EGFR KnockoutATG002B-1VL: A549 EGFR LowATG002C-1VL: A549 EGFR High

Features and Benefits

The A549 EGFR antigen panel cell lines feature EGFR expressed at varying levels (KO, low, and high). These cell lines enable researchers to screen and evaluate the efficacy and potency of EGFR-targeted immunotherapies in a biologically-relevant cellular research model.

General description

This product is a panel of three (3) genetically modified cell lines involving the EGFR gene. The panel consists of a EGFR knockout cell line (EGFR^-/-) as well as a EGFR^low and a EGFR^high expression cell line. The EGFR^-/- cell line was generated using CompoZr^™ Zinc Finger Nuclease technology, and the two EGFR expression cell lines were generated using MISSION^® lentivirus. The EGFR^low and EGFR^high cell lines express EGFR at low and high levels relative to the EGFR^-/- cell line (EGFR^high > EGFR^low > EGFR^-/-) as measured via fluorescence-activated cell sorting (FACS). As a comparison, wild type A549 cells demonstrate EGFR^medium expression as measured via FACS. The STR profile of these cell lines match that of its parental cell line - ECACC catalog number 86012804.

Legal Information

Combigel is a trademark of Sigma-Aldrich Co. LLC

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

MISSION Use Licenses AgreementZFN Use Licenses Agreement

Quality

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, and short terminal repeat (STR) analysis for cell line identification.